Production of Bovicin HC۵ in two strains of E. coli, Rosetta gami and Rosetta plysS

  • سال انتشار: 1400
  • محل انتشار: بیست دومین کنگره میکروب شناسی ایران (مجازی)
  • کد COI اختصاصی: MEDISM22_042
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 146
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نویسندگان

Manizheh Dastjanifarahani

Master of Science, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran,Iran

Zahra Hajihassan

Assistant Professor, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran,Iran

چکیده

Background and Aim : Introduction: Bacteriocins are antimicrobial peptides that are produced by ribosomes in bacteria and some archaea. Evidence suggests that these peptides could be used to treat cancer disease. Bovicin HC۵ is a bacteriocin produced by Streptococcus bovis HC۵ and was used in the treatment of liver cancer. Objective: The expression of recombinant bovicin HC۵ was performed for the first time in two bacterial strains of E. coli, Rosetta gami, and Rosetta plysS. Methods : The cDNA of Bovicin HC۵ was cloned in the pET۲۱a vector. For protein expression, IPTG with ۰.۵ and ۱ mM concentrations was used. Also, cultivation temperature of ۳۵ °C and post-induction times of ۲, ۴, and ۸ hours were selected for incubation. For protein expression analysis, the dot blot method was used and then the data were quantified by ImageJ software.Results : : The highest expression level, ۲۰.۴۹%, in the Rosetta gami strain was achieved at ۰.۵ mM IPTG and a post-induction time of ۴ hours. Also, the maximum expression rate, ۲۱.۵۰%, was observed in Rosetta plysS at ۰.۵ mM IPTG and a post-induction time of ۸ hours.Conclusion : According to the results, both strains had appropriate protein expression levels.

کلیدواژه ها

bovicin HC۵, E. coli, protein expression

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