Isolation and identification of bacteriophage affecting Pseudomonas aeruginosa isolated from Patients with cystic fibrosis (CF)

Background and Aim : Now days, due to the increasing of antibiotic resistance in Pseudomonas aeruginosa and the global decrease in the effectiveness of antibiotics treatment of bacterial infections, a novel approach has been developed to use bacteriophages in treatment of infections. Pseudomonas aeruginosa is an opportunistic hospital bacterium that causes acute infections of Respiratory system in patients with cystic fibrosis, which due to appearance of antibiotic-resistant strains in this bacterium, phage therapy is an effective approach to control this phenomenon. Methods : Initially, from the hospital wastewater, Bacteriophage was isolated by double layer method and also purified and enriched by the same method. After titration and determination of phage host spectrum by spot test, Phage stability assay against different temperatures and pH values, and determination of phage absorbance time to bacteria by double layer method has been done. Phage morphology was determined by TEM electron microscopy. Phage was used for Prevention of biofilm formation by titration assay with microplate. Results : For isolation, transparent areas of plaque indicate the presence of phage in the wastewater sample and the ability of bacteria for lysis, which after purification and enrichment, TEM images indicate that phage belongs to Myoviridae family. Isolated phage had limited host spectrum and had good function in temperature range of -۲۰ ° C to ۵۰° C and the pH value range of ۴ to ۱۰.Conclusion : According to the effectiveness and the desired results in all tests, it can be said that Isolated phage against Pseudomonas aeruginosa is effective and can be used for treatment of Infections caused by this bacterium.

کلیدواژه ها:

Pseudomonas aeruginosa ، cystic fibrosis ، bacteriophage ، Myoviridae