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Study of fosfomycin and colistin resistant in accordance to ESBL production among E. coli isolates from UTI after kidney transplantation

سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 148
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شناسه ملی سند علمی:

MEDISM22_002

تاریخ نمایه سازی: 8 مهر 1400

چکیده مقاله Study of fosfomycin and colistin resistant in accordance to ESBL production among E. coli isolates from UTI after kidney transplantation

Background and Aim : The aim of this study was phenotypic and molecular evaluation of fosfomycin and colistin resistant in accordance to ESBL production among E. coli isolates from UTI after kidney transplantation. Methods : 60 E. coli isolates from urine samples of kidney transplant patients with UTIs from 3 different Kidney transplant centers in Tehran were collected during 2018-19. Antimicrobial susceptibility test(AST) was done based on the CLSI 2018. Minimum inhibitory concentration (MIC) of fosfomycin was performed by E-test. Further ESBL phenotypic screening by double disk synergy test(DDST) was evaluated. Molecular survey of ESBL genes ( including; CTX-M, TEM, SHV), some of fosfomycin resistance (uhpT, murA) genes and mcr-1 gene as plasmidic colistin resistance were detected by PCR after DNA extraction. As control, K. pneumonia ATCC 700603, a fosfomycin resistant and a colistin resistant E.coli isolates which were bestow from Dr. C. Giske (Karolinska, Sweden) were evaluated simultanously. Sequencing was done by Bioneer Korean company and further analysis and blasting was done in data bank/NCBI.Results : Based on AST, highest susceptibility was to: doripenem, ertapenem (100%) and Imipenem 95%, and the highest resistant rate was to: ampicillin (86%), cefotaxime (80%), cefazolin and cefpodoxime (77%). Of 60 E. coli isolates, 27(45%) were associated with multidrug resistance (MDR) phenotype. Only (3.3%) of E. coli isolates showed intermediate resistant (MIC 128µg/ml) to fosfomycin based on the E- test. By DDST, 46% of isolates were identified as ESBLs producer. The frequency of ESBL genes were: blaTEM (54%), blaCTX-M (51%) and blaSHV (40%) and mcr-1 (3.3%) respectively by PCR. Mutation in different points of uhpT and murA genes were detected after sequencing analysis. Conclusion : Frequency of fosfomycin resistant was not high in this study, but, coexistence of ESBL and fosfomycin plasmidic resistance genes in E. coli isolates among kidney transplant patients, show the importance of awareness among infection-control practitioners and physicians during prescription. Also, colistin is not a recommended antibiotic for treatment and during AST for E. coli isolates by CLSI. But detection of mcr-1 gene among 3.3% of isolates is alarming because it can be exchange between different bacteria and may increase globally soon.

کلیدواژه های Study of fosfomycin and colistin resistant in accordance to ESBL production among E. coli isolates from UTI after kidney transplantation:

نویسندگان مقاله Study of fosfomycin and colistin resistant in accordance to ESBL production among E. coli isolates from UTI after kidney transplantation

Mojdeh Hakemi-Vala

Department of Microbiology,Medical school, Shahid Beheshti University of Medical Sciences Tehran-Iran

Atefeh Najafikhah

Department of Microbiology, Medical school, Shahid Beheshti University of Medical Sciences Tehran-Iran

Shiva Samavat

Department of Adult Nephrology, School of Medicine, Shahid Labbafinezhad Hospital, Shahid Beheshti University of Medical Sciences Tehran -Iran

Mohammad Javad Nasiri

Department of Microbiology, Medical school, Shahid Beheshti University of Medical Sciences Tehran-Iran

Ghazaleh Talebi

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Neda Souri

Department of Microbiology, Medical school, Shahid Beheshti University of Medical Sciences Tehran-Iran