Morphological and Molecular Investigation of Anaplasma Infection in Dromedary Camel (Camelus dromedarius) in Bushehr Province, Iran
- سال انتشار: 1400
- محل انتشار: فصلنامه طب دامی ایران، دوره: 15، شماره: 3
- کد COI اختصاصی: JR_IJVM-15-3_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 243
نویسندگان
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran- Ira
چکیده
BACKGROUND: Anaplasmosis is caused by an obligate intracellular, gram-negative microorganism, which be-longs to the family Anaplasmatacea and can be transmitted by ticks and other arthropods. OBJECTIVES: The present study aimed to investigate the status of Anaplasma spp. infection by microscopy and molecular methods in dromedary camels in Bushehr province, Iran. METHODS: A total of ۱۳۹ blood samples were collected from dromedary camels in Bushehr province. Giemsa staining and nested-polymerase chain reaction (PCR) were conducted to detect Anaplasma infection in the drome-dary camels. RESULTS: We found that ۲۷ (۱۹.۴%) out of the total ۱۳۹ blood samples were suspected for the presence of Ana-plasma spp. by morphological study. The PCR and nested-PCR sequencing results showed ۱۱۱ (۸۰%) and ۱۳۴ (۹۶%) samples positive for Anaplasma spp. and BLAST search in NCBI GenBank presented ۱۰۰% identity with Candidatus Anaplasma camelii. CONCLUSIONS: The molecular results presented the high frequency of Candidatus Anaplasma camelii in camels, in Bushehr city.کلیدواژه ها
Anaplasma, Candidatus Anaplasma camelii, Dromedary camel, Molecular study, Nested-PCRاطلاعات بیشتر در مورد COI
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