Synergistic effects of Coxsackievirus oncolytic virus in combination with copper nanoparticles, hyperthermia and radiation on CT۲۶ cell proliferation (malignant colorectal carcinoma)
سال انتشار: 1400
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 125
متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
ICNNA02_230
تاریخ نمایه سازی: 4 مهر 1400
چکیده مقاله:
Background & Aims: Today, due to changes in nutritional pattern, cancers of the gastrointestinal tract, especially the colon is one of the most common causes of death. Drug resistance of many cancers during treatment with chemotherapy drugs has led researchers to use innovative multifactorial therapies. The aim of this study was to investigate the Synergistic effects of Coxsackievirus oncolytic virus in combination with copper nanoparticles, hyperthermia and radiation on CT۲۶ cell proliferation (malignant colorectal carcinoma) as a new multifactorial method.Background & Aims: Today, due to changes in nutritional pattern, cancers of the gastrointestinal tract, especially the colon is one of the most common causes of death. Drug resistance of many cancers during treatment with chemotherapy drugs has led researchers to use innovative multifactorial therapies. The aim of this study was to investigate the Synergistic effects of Coxsackievirus oncolytic virus in combination with copper nanoparticles, hyperthermia and radiation on CT۲۶ cell proliferation (malignant colorectal carcinoma) as a new multifactorial method.Methods: In this study, after culturing CT۲۶ cells (malignant colorectal carcinoma) in ۱۳ T۲۵ flasks, they were combined with CuO nanoparticles (۱۰۰ μg/ml ،), Coxsackievirus oncolytic virus (MOI۴۰), Radiation (cGy۲۰۰) and hyperthermia (۴۱ Degrees Celsius). After determining the uptake of CuO nanoparticles to confirm the anti-cancer effects of the above factors (MTT), percentage of apoptosis, production of reactive oxygen species (ROS), release of lactatedehydrogenase (LDH), activity level Caspase ۸ and ۹ were measured. Methods: In this study, after culturing CT۲۶ cells (malignant colorectal carcinoma) in ۱۳ T۲۵ flasks, they were combined with CuO nanoparticles (۱۰۰ μg/ml ،), Coxsackievirus oncolytic virus (MOI۴۰), Radiation (cGy۲۰۰) and hyperthermia (۴۱ Degrees Celsius). After determining the uptake of CuO nanoparticles to confirm the anti-cancer effects of the above factors (MTT), percentage of apoptosis, production of reactive oxygen species (ROS), release of lactate dehydrogenase (LDH), activity level Caspase ۸ and ۹ were measured.
کلیدواژه ها:
نویسندگان
Hadi Esmaeili Gouvarchin Ghaleh
Applied Virology Research Center, Baqiyatallah University of Medical sciences, Tehran, Iran
Bahman Jalali Kondori
Gastroenterology and Liver Disease Research Center, Baqiyatallah University of Medical sciences, Tehran, Iran