Association of miR-۱۴۶a and miR۱۹۶a۲ genotype with susceptibility to idiopathic recurrent pregnancy loss in Iranian women: A case-control study

Background: Recurrent pregnancy loss (RPL) is the most common complaint of pregnancy in females with a prevalence of ۵%. Numerous documents have shown that single nucleotide polymorphisms are able to change miRNA transcription and/or maturation, which may alter the incidence of disorders such as RPL. Objective: To assess the relationship of miR-۱۴۶aC > G (rs۲۹۱۰۱۶۴) and miR-۱۹۶a۲T > C (rs۱۱۶۱۴۹۱۳) with RPL susceptibility in Iranian women. Materials and Methods: Blood samples were collected from ۲۱۴ women who had experienced at least two consecutive spontaneous miscarriages (case) and ۱۴۷ normal individuals without a history of miscarriage (control). MiR-۱۴۶aC > G and miR-۱۹۶a۲T > C genotypes were evaluated via the restriction fragment length polymorphism technique. Results: The genotypes incidence did not show a significant difference in pre-miR-۱۴۶aC > G polymorphism CC vs CG + GG (p = ۰.۸۵۴; OR = ۰.۹۳۳; ۹۵% CI) and CC + CG vs GG (p = ۰.۲۸۲; OR = ۱.۴۵۴; ۹۵% CI). Also, no significant difference was observed between pre-miR-۱۹۶a۲T > C polymorphism TT vs TC + CC (p = ۰.۸۶۲; OR = ۰.۹۳۸; ۹۵% CI) and TT + TC vs CC and (p = ۰.۲۹۱; OR = ۱.۴۶۲; ۹۵% CI) in both the case and control groups. Conclusion: The results showed that although the distribution of miR-۱۴۶aC > G and miR-۱۹۶a۲T > C was different between the unknown RPL and control groups, these variances were not statistically significant.

کلیدواژه ها:

RPL ، miR-۱۴۶a ، miR۱۹۶a۲ ، Polymorphism ، RFLP. ، RPL ، miR-۱۴۶a ، miR۱۹۶a۲ ، پلی­مورفیسم