Investigation of DNA Integration into Reproductive Organs Following Intramuscular Injection of DNA in Mice
- سال انتشار: 1391
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 1، شماره: 1
- کد COI اختصاصی: JR_RBMB-1-1_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 199
نویسندگان
Razi Vaccine & Serum Research Institute, Mashhad, Iran
Biochemistry Department, Payam-e-Noor University, Mashhad, Iran
Biochemistry Department, Payam-e-Noor University, Mashhad, Iran
Razi Vaccine & Serum Research Institute, Mashhad, Iran
چکیده
Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. The use of plasmid DNA vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of DNA vaccines exist, such as the possibility of integration into the host genome, and elicitation of adverse immune responses. Methods: In this study, we examined the potential integration and bio-distribution of pcDNA۳.۱+PA, a new vaccine candidate with GenBank accession # EF۵۵۰۲۰۸, encoding the PA۶۳ gene, in reproductive organs of mice; ovaries and uterus in female, and testis in male. Animals of both sexes were injected intramuscularly with pcDNA۳.۱+PA. Host genome integration and tissue distribution were examined using PCR and RT-PCR two times monthly for six months. Results: RT-PCR confirmed that pcDNA۳.۱+PA was not integrated into the host genome and did not enter reproductive organs. Conclusions: This finding has important implications for the use of pcDNA۳.۱+PA plasmid as a vaccine and opens new perspectives in the DNA vaccine area.کلیدواژه ها
DNA, Intramuscular injection, Integration, Mice, Reproductive organsاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.