Determination of Haptoglobin Genotype in an Iranian Population with Idiopathic Generalized Epilepsy
- سال انتشار: 1394
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 3، شماره: 2
- کد COI اختصاصی: JR_RBMB-3-2_001
- زبان مقاله: انگلیسی
- تعداد مشاهده: 228
نویسندگان
Department of Biology, Al Zahra University, Tehran, Iran
Department of Biology, Al Zahra University, Tehran, Iran
Department of Biology, Al Zahra University, Tehran, Iran
Department of Biology, Al Zahra University, Tehran, Iran
Cellular and Molecular Biology Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran - Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Iranian Epilepsy Association, Tehran, Iran
Cellular and Molecular Biology Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran - Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
Background: Haptoglobin (Hp) is a plasma α۲-sialoglycoprotein that contains alpha and beta chains. It displays in three common phenotypes, Hp۱-۱, Hp۲-۱, and Hp۲-۲. Proteins expressed by polymorphic genes have grossly different molecular sizes resulting in different diffusion rates in the brain. Haptoglobin expressed by the Hp۲-۲ genotype has lower hemoglobin-binding capacity than Hp۱-۱ or Hp۲-۱ and is associated with idiopathic generalized epilepsy. Methods: To determine polymorphism in haptoglobin genes in patients with idiopathic generalized tonic-clonic seizures, ۴۲ men, ۴۲ women, and ۵۰ controls were selected for this study. Genomic DNA was extracted from blood and studied by polymerase chain reactions (PCR). Results: The amplified fragments for the Hp۱-۱ and Hp۲-۲ genotypes were ۱۷۵۷ and ۳۴۸۱ base pairs (bp) respectively, and the Hp۲-۱ genotype had both fragments, in addition to a ۳۴۹-bp fragment. The distribution of the three major Hp phenotypes in epilepsy patients was ۲۸.۶ (۱-۱), ۳۸.۱ (۲-۱), and ۳۳.۳% (۲-۲) in the men, and ۳۱ (۱-۱), ۴۰.۵ (۲-۱), and ۲۸.۶% (۲-۲) in the women. The distribution of Hp genotypes in controls was ۲۲ (۱-۱), ۴۰ (۲-۱), and ۳۸% (۲-۲). Conclusion: We show that all Hp genotypes participate in idiopathic generalized epilepsy.کلیدواژه ها
Epilepsy, Haptoglobin, Iranاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.