Mesenchymal Stem Cells Differentiate to Endothelial Cells Using Recombinant Vascular Endothelial Growth Factor –A
- سال انتشار: 1397
- محل انتشار: مجله گزارش های بیوشیمی و زیست شناسی مولکولی، دوره: 6، شماره: 2
- کد COI اختصاصی: JR_RBMB-6-2_005
- زبان مقاله: انگلیسی
- تعداد مشاهده: 278
نویسندگان
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran-Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
National Institute for Genetic Engineering and Biotechnology, Tehran. Iran.
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran-Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran-Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
چکیده
Background: Vascular endothelial growth factor-A (VEGF-A), an endothelial cell-specific mitogen produced by various cell types, plays important roles in cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of mesenchymal stem cells (MSCs) to endothelial cells (ECs). Methods: VEGF-A was expressed in E. coli BL۲۱ (DE۳) and BL۲۱ pLysS competent cells with the pET۳۲a expression vector. Recombinant VEGF-A protein expression was verified by SDS-PAGE and western blotting. Mesenchymal stem cell differentiation to ECs in the presence of VEGF-A was evaluated by flow cytometry and fluorescence microscopy. Results: Recombinant VEGF-A was produced in E. coli BL۲۱ (DE۳) cells at ۰.۸ mg/mL concentration. Expression of CD۳۱ and CD ۱۴۴ was significantly greater, while expression of CD۹۰, CD۷۳, and CD۴۴ was significantly less, in MSCs treated with our recombinant VEGF-A than in those treated with the commercial protein (p < ۰.۰۵). Conclusions: Recombinant VEGF-A expressed in a prokaryotic system can induce MSCs differentiation to ECs and can be used in research and likely therapeutic applications.کلیدواژه ها
Cell differentiation, Endothelial cell, Mesenchymal stem cell, Vascular Endothelial Growth Factor Aاطلاعات بیشتر در مورد COI
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