Comparison of some biochemical traits in two transgenic lines of tobacco in potted conditions

The tobacco plant (Nicotiana tabacum) belongs to the Solanaceae family and it is used as a model system in the laboratory. The rolC gene induce the hairy root disease in transformed plants which its expression in these plants stimulates rhizogenesis and cause the morphological and biochemical alterations. Studies conducted on the tobacco plant have shown that the plant naturally has a homologous sequence of the rolC gene of Agrobacterium rhizogenesis, called trolC. This study was aimed to investigate some biochemical characteristics in two lines of tobacco transgenic plants, rolC and trolC, under pot culture conditions. The experiments were performed in ۳ replications for each sample and data were finally analyzed by SPSS ۱۶.۰ software. Excel ۲۰۱۳ software was also used to draw the graphs. The results showed that the difference between control samples and transgenic plants was significant at the ۵% probability level. Activity of antioxidant enzymes such as peroxidase and ascorbate peroxidase was increased in induced transgenic plants, while the activity of catalase was decreased and the superoxide dismutase enzyme did not show any significant difference between control and induced plants. Also, in the case of MDA content, there was no significant differences between the control and transgenic plants. The H۲O۲ content was increased in transgenic plants in comparison to non-induced ones. Since the activity of the catalase enzyme decreased and H۲O۲ content increased in induced plants compared to non-induced ones, it is inferred that the stress management has been done through non-enzymatic pathway. The interesting point of this study was the similar function between trolC and rolC transgenic plants, and this indicates that the functional characteristics of this gene were protected after transferring to the tobacco plants during the evolution

کلیدواژه ها:

Nicotiana tabacum ، Agrobacterium rhizogenes ، rolC genw ، trolC gene