Evaluation of effective parameters on menthol extraction from Semnan native peppermint plant using Hydro-distillation method

One of the most famous sources of medicinal plants containing essential oils (EOs) is the peppermint plant, which belongs to the Lamiaceae family. Due to having more menthol than all branches of the Lamiaceae family, it has received more attention. Peppermint plant has many properties such as: antimicrobial, antiviral, antioxidant, anti-cancer, anticoagulant and anti-allergy and is widely used in various medical, pharmaceutical, food, cosmetic and perfumery industries . in this study, from Semnan native peppermint plant for extraction of more menthol according to the parameters affecting the extraction such as solid to solvent ratio (grams of dry sample / amount of solvent in ml) equal to ۱:۱۰, ۱:۱۵ and ۱: ۲۰, thermal power (kW) ۶, ۸ and ۱۰ and average particle size (mm) ۰.۵, ۰.۷۵ and ۱ have been investigated. The EOs was extracted from the peppermint plant by the most common method of industrial distillation, Hydro-distillation. The advantage of using this method is simplicity in setting up and not using solvent. GC and GC-MS have been used to identify the EOs compounds. Peppermint was prepared from Hassan-Abad area, located ۵ km southwest of Semnan in June ۲۰۱۹. Samples were stored in high density polyethylene bags after ۵ days of drying at ۳۰ to ۴۰ ° C. Crush ۵۰ g of the dried sample to the desired size and mix it with deionized distilled water and ۵ g of boiling stone and after placing it in the balloon of the Clevenger device, the test is performed. The results showed that in the ratio of solid to solvent ۰.۰۵ g / ml, heat capacity of ۹ kW and average particle size of ۰.۵ mm, the amount of EOs production was ۱۵.۸۴۹۴%. The six main constituents of peppermint EOs are ۴۴.۶۰% menthol, ۲۱.۹۲% menthone ۶.۲۷% isomenthone, ۴.۳۷% (E)-Caryophllene, ۳.۷۹% Germacrene-D and ۳.۷۰% of ۱,۸-Cineole.

کلیدواژه ها:

Medicinal Plants ، Essential Oils (EOs) ، Clevenger ، GC ، GC-MS