Effect of explant size and culture medium on meristem culture of strawberry (Fragaria ananassa)

سال انتشار: 1399
محل انتشار: بیست و یکمین کنگره ملی و نهمین کنگره بین المللی زیست شناسی ایران
کد COI اختصاصی: BIOCONF21_1025
زبان مقاله: انگلیسی
تعداد مشاهده: 355

نویسندگان

Msc student, Plant Biotechnology Department,University of Tabriz Iran

Associate professor, Agricultural Biotechnology Research Institute of Iran, Tabriz.

چکیده

Nowadays, plant tissue culture is used as a useful tool for induction a new genetic variation to crops breeding as well as to produce disease-free plants. Meristem culture is one of the most important ways of producing virus-free plants. Given the importance of strawberry production and viral diseases damage in plant production, it is necessary to implement proper management strategy in the production of virus-free plant materials. This study was carried out in the plant biotechnology laboratory of Tabriz University with the aim of optimizing the meristem culture of strawberry (Fragaria ananassa) CV,Gavita and investigating the factors affecting it. In the first experiment to investigate the effect of meristematic dome size on meristem culture, three different explant including meristematic dome, meristematic dome with one primordial leave and meristematic dome with two primordial leaves separated and in cultured in MS supplemented with ۱ mg/l BAP and ۰.۲ mg/l IAA. The second experiment was conducted to investigate the effect of liquid, semi-solid and solid MS medium on meristem regeneration. All mediums supplemented with ۱ mg/l BAP and ۰.۲ mg/l IAA. All experiments were performed in a completely randomized design with five replications. Traits evaluated included percentage of survived meristem, number of regenerated shoots, and length of shoots. The results showed that there was a significant difference between the meristem dome size and the number and length of shoots obtained from meristem.The best results obtained with the meristematic dome with two leaves. Results showed that there was a significant difference between mediums in meristem induction and the best result obtained from semi-solid medium where all meristems were regenerated and produced more shoots with highest length.

کلیدواژه ها

Gavita, meristematic dome, virus, MS

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.