Regeneration of Stevia rebaudiana Bertoni plant in somatic embryogenesis using Multi-walled Carbon Nanotube

Stevia rebaudiana (Bert.) belonging Asteraceae family is one of the most important medicinal plants. Due to the incompatibility of the plant, somatic embryogenesis is valuable. Nowadays, carbon nanotubes (CNTs) can give new possibilities for modern applications in the field of biotechnology and agriculture. In the study, embryogenesis and the possible impact of MWCNTs on embryogenesis have been investigated. Observations and results indicate that the combination and hormonal concentration used is very important.The highest rate of embryogenesis in light was observed at ۱ mg l-۱ ۲,۴-D with ۰.۲ mg L-۱ kin and BA hormones in callus, and the highest rate of embryogenesis in light was observed in dark at ۱ and ۲ mg L-۱ ۲,۴-D hormone with ۰.۲ mg l-۱ kin in callus. Also, the data indicated that MWCNTs had a positive effect on the fresh and dry weight of calluses and the highest fresh and dry weight observed in ۵۰۰ μg l-۱ treated group. while, it had a negative effect on the rate of embryogenesis, and in MWCNTs -treated groups, decreased compared to the control, and causing cell death. In this research, as in previous studies, it was found that, duration and particle size of used MWCNTs, and as well as the stage of plant growth, the main cause and decisive in creating phytotoxicity, or positive of MWCNTs on growth, callus formation and embryogenesis in plants.

کلیدواژه ها:

Asteraceae family ، Callus ، somatic embryogenesis ، Carbon Nanotube ، Tissue Culture