Genetic diversity and population structure of Trifolium resupinatum L. using CDDP method

Trifolium resupinatum L. (Fabaceae) is a cosmopolitan plant and one of the most remarkable resources for grazing, nitrogen fixation, and also soil texture improvement in natural environments. Various molecular markers evaluated the genetic variation and remaining genetic parameters of different taxa worldwide. Accordingly, the current study supposed to check the capability of CDDP method to exhibit the genetic variation and polymorphism of Trifolium resupinatum cultivars throughout Iran, and thus the number of ۱۵ cultivars were collected. In our case, DNA was extracted using modified CTAB methodology. A total of ۲۴۳ scoreable bands were generated based on ۲۰ primers. High level of polymorphism (۹۴.۶۵%) was revealed within the studied cultivars. WRKY-R۱ indicated the lowest polymorphism, while WRKY-R۱, ABP۱-۱, WRKY-F۱ and ERF۳ genetic primers were displayed the highest variation. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis and Principle coordinate analysis (PCoA) was constructed based on Horn coefficient similarity using PAST software. The number of population structures (K) was detected by STRUCTURE and STRUCTURE harvester. The number of clusters was determined two, and the cultivars belonging to south indicated more similarity and classified into the same group. PCoA result was also in agreement with UPGMA cluster analysis.

کلیدواژه ها:

Biodiversity ، Cultivar ، Persian clover ، Polymorphism ، Population structure