Heliotrine extraction and detoxification by bacterial derived FAD-dependent oxidoreductase
- سال انتشار: 1399
- محل انتشار: بیست و یکمین کنگره ملی و نهمین کنگره بین المللی زیست شناسی ایران
- کد COI اختصاصی: BIOCONF21_0865
- زبان مقاله: انگلیسی
- تعداد مشاهده: 158
نویسندگان
Dep. for Biotechnology, Faculty of New Sciences and Technologies, Semnan University, IRAN
Dep. for Biotechnology, Faculty of New Sciences and Technologies, Semnan University, IRAN
Agricultural and Natural Resources Research and Education Centre of Semnan province (Shahrood)
Dep. for Biology, Faculty of Basic Science, Semnan University, Iran
چکیده
Alkaloids are plant secondary metabolites or toxins produced by plants. Pyrrolizidine alkaloids (PA), with toxic effects on human, are widely distributed in plants. Hepatoxic pyrrolizidine alkaloids are one of the most important causes of human disease in the prevalence of liver poisoning. To remove PA from feed, enzymatic biotransformation currently is concerned. Here, heliotrine a dominant PA in Gole Gav-Zaban herbal tea, from Echium amoenum was investigated. The petals were extracted with methanol at ۶۰°C by soxhlet extractor. The impurities were removed applying diethyl-ether and ethyl-acetate, the resin debris pelleted by NaCl. The method ended by adding chloroform to collect heliotrine. The purification of heliotrine was done by silica gel column and a concentration gradient in chloroform-methanol. FAD-dependent oxidoordoxase derived from Pseudomonas aeruginosa ۲۷۸۵۳ ATCC Structurally similar to human-specific FMO۳, effective on the metabolism of toxins in the liver was used to oxidize heliotrine as a pyrrolizidine alkaloid precursor. Enzyme activity of Fmo۵p was assayed photometrically. reaction was set up in a total volume of ۲۰۰ μl, ۰.۱-۱ μm the final concentration of the recombinant enzyme (periplasmic proteins, ۵۰ mM Tris-HCl buffer pH=۸ and ۱۰۰ μM NADPH at ۲۵°C in Quartz covet. The reaction was followed by the decrease of NADPH absorption at ۳۴۰ nm with an UV/Visible Spectrophotometer in ۳۰ second steps until the change stops. Control experiments without enzymes were prepared. The difference in light absorption at ۳۴۰ nm for the test sample was ۰.۴۰۴ and for the control reaction was ۰.۰۲۷ over ۵ minutes, which was a confirmation of the oxidative activity of Fmo۵p enzyme to be used to detoxify heliotrine. This enzyme is introduced as a drug design candidate. Detoxification effects of this oxidoreductase on other types of pyrrolizidine alkaloids is suggested in the continuation of present study.کلیدواژه ها
Echium amoenum, FAD-dependent oxidoreductase, Heliotrine, Pyrrolizidineمقالات مرتبط جدید
- مروری بر نقش گیاه آرتمیزیا (Artemisia annua) در درمان مالاریا از طب سنتی تا طب جدید
- مروری بر گیاهان دارویی ایران با بیشترین همپوشانی مواد موثره جهت توسعه درمانهای ترکیبی
- اسفرزه (Plantago ovata Forsk) موثر در درمان کولیک شنی در اسب
- تحلیل عوامل موثر بر کاربرد گیاهان دارویی در درمان بیماری کووید-۱۹
- تاثیر روشهای خشک کردن بر کمیت و کیفیت اسانس گیاه دارویی گزنه
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.