The evaluation of inhibitory effects of secretome from mesenchymal stem cells on invasion of pancreatic cancer cells by By analyzing the expression of Vimentin gene

They can differentiate into specific cell types in vitro and in vivo and have a tendency to acquire tissue specific characteristics when co-cultured with specialized cell types like cancer cells Invasion is the first step in the metastatic cascade, when tumor cells acquire the ability to move, penetrate into the surrounding tissue and enter lymphatic and blood vessels in order to disseminate. The aim of this study was to determine the expression of Vimentin gene, a suppressor of cancer growth. Any dysfunction of this gene leads to cancer progression and metastasis. When the production of this protein decreases, cell-to-cell adhesion decreases and cell motility increases. This allows cancer cells to cross the basement membrane and invade nearby tissues. In this study, we used co-cell culture for cancer cells and stem cells at different times using a ۶-cell two-story plate with a diameter of ۰.۴ μm (Transwell) and using qRT-PCR expression of Vimentin size gene to found that it inhibited metastasis or EMT by controlling Vimentin. The results showed that reducing the regulation of vimentin expression in pancreatic cancer cells by transfection with antisense-Vimentin resulted in a significant reduction in tumor cell motility and invasive activity. Furthermore, the expression of E‐cadherin was inversely associated with expression of Vimentin. Our results suggest that Vimentin affects pancreatic cancer cells motility and invasiveness.

کلیدواژه ها:

Pancreatic cancer ، gene expression ، Vimentin