Evaluation and comparison of Newcastle disease virus F protein to determine immunogenicity and pathogenicity of isolates in Eurasia

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 148

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شناسه ملی سند علمی:

BIOCONF21_0817

تاریخ نمایه سازی: 7 شهریور 1400

چکیده مقاله:

Avian paramyxovirus serotype ۱ (APMV-۱) is the causative of Newcastle disease in birds. The disease has been reported in many organisms, including humans, but its acute form is found in birds. Newcastle virus has a single-stranded RNA genome that encodes proteins such as hemagglutinin-neuraminidase (HN), nucleocapsid protein (NP), phosphoprotein (P), and fusion protein (F). One of the most important factors in the pathogenicity of this virus is F protein. The precursor of this protein in the infected cell must be cleaved into two mature proteins by proteolytic cleavage. This protein also promotes high immunogenicity in infected organisms, which is a target for the development of vaccines against the virus. In this study, the F protein of Newcastle disease virus isolates from different species such as camels, owls, pigeons, and chickens in the vast region of Eurasia, where many animal species migrate and interact, was examined. F protein sequences of different strains of Newcastle disease virus isolated from Eurasia were extracted from GenBank®. These sequences were then examined in the amino acid region of ۱۱۲ to ۱۱۷, which is the site of proteolytic cleavage. Immunogenicity and immunogenic epitopes of these sequences were examined by VaxiJen v۲.۰ tool and the IEDB website. The results showed that the cleavage site of the F protein precursor shows six diverse motifs. From these six motifs, four are specific to pathogenic strains and two are related to non-pathogenic strains. Twenty-six pathogenic samples and six non-pathogenic samples were identified from the studied sample pool. The immunization of the sequences was in the range of ۰.۴۹ to ۰.۵۶ with a threshold of ۰.۴ for viral proteins, indicating low immunogenicity of this protein alone. Also, high-scored common epitopes were identified among circulating F protein sequences of pathogenic and non-pathogenic strains. These epitopes will be used for further studies in vaccine design.

کلیدواژه ها:

Immunogenicity ، Newcastle disease ، epitope ، F protein cleavage site

نویسندگان

Maryam Barkhordari

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Mohammad-Hosein Khani

Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran