Inhibition of α-Amylase and α-Glucosidase from Caucasotachea lencoranea by flavonoids extract of Arctium lapa L.

Citrus brown snail is one of the citrus pests in the north of Iran and the identification of digestive enzymes of this species can play an important role in controlling this pest. α-amylase and α-glucosidase enzymes were extracted from the gastrointestinal tract at ۴ ° C after homogenization and centrifugation. The activity of α-amylase and α-glucosidase were ۰.۲۷۸ and ۰.۴ enzyme units per ml, respectively. Also, Km and Vmax values for amylase were calculated to be ۰.۱۳ mM and ۰.۰۶۲ mM / min, respectively, and for alpha glucosidase, ۱.۵ mM and ۰.۰۲۸ mM / min, respectively. Arctium lapa L. has been used in human medication due to its anti-inflammatory, liver protection, antimicrobial and antifungal effects. Flavonoid extract of this plant was prepared and activity of the enzymes were measured in its vicinity. The flavonoid content of the extract was measured by aluminum chloride method. This amount was equal to ۲.۱ mg equivalent to quercetin per ml of extract. Acarbose was used as a specific inhibitor. The α-amylase and α-glucosidase were inhibited by Arctium lapa flavonoids and the IC۵۰ levels were determined: ۰.۱۶ mg / ml and ۱.۷ mg / ml, respectively. Due to the low value of IC۵۰ and the results of our experiments, the flavonoid extract of this plant can be suggested as a suitable alternative for agricultural or therapeutic uses of chemical compounds.

کلیدواژه ها:

Kinetic Parameters ، Partial Purification ، Citrus Pest ، Diabetes