Study the effect of oil extraced Pseodomonas aeruginosa to stimulate immune system in Arabidopsis thaliana

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 118

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شناسه ملی سند علمی:

BIOCONF21_0796

تاریخ نمایه سازی: 7 شهریور 1400

چکیده مقاله:

In this research, the Pseudomonas aeruginosa which was isolated from oil, initially was grown in MS medium for ۲۴ hours. The bacterium was harvested and inoculated to Arabidopsis thaliana seedling in ۰. ۰۰۲ optical density. It is observed that inoculated plants have lower growth compared to control plants. In order to determine whether the dead Pseudomonas aeruginosa can stimulate defense system of A. thaliana, the dead bacterium was inoculated to Arabidopsis plants. To the control plants only inoculation buffer without any bacterium was added. One hour after inoculation, it was seen that the dead bacterium can enhance the transcription of (PR-۱) PATHOGENESIS-RELATED GENE ۱. Furthermore, it is observed that the dead bacterium can enhance the transcription of BRI۱-ASSOCIATED RECEPTOR KINASE ۲۴ hours after inoculation. Transcription of PR-۱ and BAK-۱ genes is regarded as the most importnat factors that their transcription induce the immune system in A. thaliana. It is observed that compared to AtPep۳ nano peptide, the dead P. aeruginosa could higher enhance the transcription of PR-۱ and BAK-۱ genes. The resutls of this study for the first time showed that the dead P. aeruginosa can enhance the defence systen in model plant A. thaliana and induce the priming effect. The application of priming effect to induce resistance againt plant diseases has great value to control important plant pathogen. It is suggested to apply this finding in important crop plants and control important plant pathogens.

نویسندگان

Mehdi Safaeizadeh

Department of Plant Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

Darioush Minae Tehrani

Department of Microbiology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran