Effect of Secretome secreted from mesenchymal stem cells cultured with colorectal cancer cells By analyzing the expression of Bax and Bcl۲ genes

Colorectal cancer is the third most common cancer and the second leading cause of cancer mortality worldwide due to the fact that mesenchymal stem cells are very similar to cancer cells in terms of speed of proliferation, studies have shown that due to the anti-cancer properties of mesenchymal stem cells, the interaction between cancer cells and mesenchymal stem cells can help treat many cancers. The aim of this study was to investigate the expression of Bax and Bcl, apoptotic genes. In this study, we are looking for the role of apoptosis in the development and treatment of cancer and focus on the mitochondrial pathway of apoptosis, the most common regulator of cell death in cancer. Studies have shown that the two Bax and Bcl۲ genes contribute to programmed cell death or apoptosis. The association and ratio of Bax to Bcl۲ also determines cell survival or death after an apoptotic stimulus. In this study, we used co-cell culture for cancer cells and stem cells at different times using a ۶-cell two-story plate with a diameter of ۰.۴ um (Transwell) and Using RT-PCR, we measured the expression of Bax and Bcl۲ apoptotic genes to find that the effects of mesenchymal stem cell scrotum on colorectal cancer cells inhibited or stimulated Bax and Bcl۲ apoptotic genes. The results showed that there was an inverse correlation between Bax and Bcl۲ expression in individual tumors and significant Bcla inversion. The highest expression level Bcl۲ and the ratio Bcl۲.Bax were associated with p۵۳ mutant immunophenotype. Therefore, we conclude that aggressiveness in colorectal tumors could be linked, in addition to proliferation, to apoptosisrelated factors.

کلیدواژه ها:

Colon cancer ، Stem cells ، Gene expression ، Bax ، Bcl۲