Studying the Cytotoxic and Genotoxic effects of Teucrium persicum on A۳۷۵ Melanoma Cancer Cells

Melanomas are malignant skin tumors originate from the transformation of the melanocytes. Teucrium genus belongs to the Lamiaceae family and the Ajugoideae subfamily, which its species are used to treat a diversity of diseases including diabetes, inflammation, hyperlipidemia, rheumatism and cancer. Teucrium persicum is an endemic plant of Iran, which is used in traditional medicine for relieve headache and abdominal pains. The purpose of this study is to investigate the cytotoxicity and genotoxicity effects of methanolic extract of T. persicum on A-۳۷۵ cells. In this study, the efficacy of different concentrations of T. persicum plant extract on A-۳۷۵ and HEK-۲۹۳ cell lines was evaluated by using MTT assay and the genotoxicity effect of T. persicum extract was studied using the micronucleus assay. The results of MTT assay showed that T. persicum extract significantly reduced viability of A-۳۷۵ cells (IC۵۰ = ۳۶.۱۳ and ۱۳μg/ml for ۲۴ and ۴۸ hours respectively) and HEK-۲۹۳ cells (IC۵۰ = ۴۹.۹۴μg/ml for ۴۸ hours). In addition, the treatment of A-۳۷۵ cells with different concentrations of T. persicum extract significantly led to micronucleus production. These results suggest that T. persicum is a plant with very potent anticancer activity.

کلیدواژه ها:

Melanoma ، T. persicum ، A-۳۷۵ cells