Investigation on feasibility of blood grouping kit Producing by gel microcolumn technique

Nowadays the determination of blood type (ABO & Rh) plays a very important role in the blood transfusion process, and every year some patients who receive blood transfusion die due to the false results of blood group typing. In old methods, technician errors and false reading of the results may cause change of the results. In this study we determined the kind of gel chromatography component and buffer which was suitable for micro-columns blood grouping test and we also found suitable and stable conditions such as anti-body concentration, PH, etc. for better agglutination reaction in gel technique. In the second part of this study, micro-columns were prepared and compared with tube blood group typing method, ۲۰۰ blood samples were used for performance evaluation and it was found that the sensitivity and specificity of micro-column method are consistent with the tube method, but the measurement of agglutination grade was slightly better in the micro-column method than tube method. According to the functional tests, the micro-columns made in this study can be produced at industrial scale and this method can be used to standardize the blood group test instead of tube method. Moreover, gel method can be accomplished by automated sampling and image processing system in order to reduce human errors while administrating blood group typing test and reading the results.

کلیدواژه ها:

agglutination ، blood group ، gel chromatography ، gel technique ، micro-column