Optimizing the conditions for thiolation of magnetic chitosan- CNT

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 132

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شناسه ملی سند علمی:

BIOCONF21_0722

تاریخ نمایه سازی: 7 شهریور 1400

چکیده مقاله:

Chitosan is a linear polysaccharide which deacetylated derivative of the biopolymer chitin for nanotechnology applications due to its unique characteristics such as the lake of nontoxicity, excellent biocompatibility, and biodegradability. Magnetic nanoparticles (MNPs) have a large specific surface area and provide rapid and easy recovery of the biocatalyst from the reaction. Carbon nanotubes (CNT) have a higher surface area for enzyme immobilization and the conjugation of enzymes with CNT can increase the stability and activity of the enzyme. This study aimed to optimize the conditions for thiolation of magnetic chitosan- CNT for enzyme immobilization. Fe۳O۴ nanoparticle was made by the CO-precipitation method in which chitosan was coated on the Fe۳O۴ during adding TPP. Then multiwalled CNT was functionalized with carboxyl groups (CNT-COOH) and cross-linking with amine groups of chitosan by glutaraldehyde. Thiolated chitosan was synthesized using different concentrations of ۰.۵, ۰.۷۵, ۱ M TGA in the presence of ۱-ethyl-۳-۳(۳-dimethylaminopropyl) carbodiimide hydrochloride (EDC). Ellman's method was used to determine the amount of thiol groups. The composites characterized by Fourier transform infrared spectroscopy (FTIR). The result showed magnetite –chitosan and magnetite- thiolated chitosan-CNT were absorbed by external magnetite and that enzyme binding to the thiol functional group of chitosan was successful. The prepared TCS could be used for biomedical applications such as drug and gene delivery, enzyme application such as enzyme immobilization for removing heavy metals from wastewater, and preparation of biosensors.

نویسندگان

Fatemeh Veysi

Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Iran.

Ali taravati

Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Iran.