The effect of citalopram as antidepressant drug on TM۴ sertoli cells

Depression, is a common chronic illness and crisis like the Coronavirus Disease ۲۰۱۹ (COVID-۱۹) pandemic may increase the current prevalence of these illness. Citalopram is a antidepressants drug, which commonly is prescribed. Among the relatively neglected and less-studied potential side effects of psychotropic drugs are impairment of sperm parameters and fertility problems among male patient. The aim of this study was to evaluate the effect of citalopram on quantitative and qualitative parameters of sperm Which is important in fertility.In this study, the antiproliferative effect of citalopram on TM۴ mouse sertoli cells at ۲۴, ۴۸ and ۷۲ at concentrations of ۰, ۱۰, ۵۰, ۱۰۰, ۱۲۰, ۱۶۰ and ۲۵۰ μM was investigated. These cells were cultured in DMEM/F۱۲, HEPES containing ۲.۵% FBS, ۵% HS and ۱% of penicillin and streptomycin antibiotics. The morphology of the cells was examined using an electron microscope. MTT result was performed to determine toxic and non-toxic concentrations. Annexin-V expression was examined by flow cytometry to determine the type of induced death on cells. Microscopic observations of citalopram-treated cells and their comparison with each other at low concentrations showed little morphological changes; however, at concentrations of ۱۲۰, ۱۶۰, and ۲۵۰ μM, certain morphological changes were observed in the treated cells. MTT results showed that increasing the concentration of citalopram significantly reduced (****p<۰.۰۰۰۱) the proliferation of TM۴ mouse sertoli cells. Also, analysis of Annexin-V expression by flow cytometry showed that the type of death induced by citalopram was apoptosis. The results of this study showed citalopram induced apoptosis in TM۴ class sertoli mouse cells at high concentrations (۱۲۰-۲۵۰ μM) and it affected the natural morphology of sertoli cells. Therefore, the possibility of infertility disorders in the use of this drug should be considered.

کلیدواژه ها:

Fertility ، Apoptosis ، Depression ، Flow cytometry ، Cell proliferation