Effects of serotonin reuptake inhibitor on aromatase gene expression in polycystic ovaries of Wistar rats

Polycystic ovary syndrome (PCOS) is a female endocrine disorder that affects ۵% to ۱۰% of women. One of the causes of PCOS is an increase in the mean plasma concentration of androgen, which is associated with impaired folliculogenesis and is controlled by FSH, LH, insulin, and androgen. The aim of this study was to evaluate the effects of serotonin reuptake inhibitor (Escitalopram) on aromatase gene expression in PCOS rats. In this study, ۳۶ female Wistar rats were divided into four groups: healthy rats, PCOS rats, treatment group during the time required for polycystic induction, and treatment group after polycystic induction. To induce PCOS, ۴ mg of Estradiol Valerate was dissolved and infused in ۰.۲ ml of olive oil. The treatment groups, which were randomly divided into ۳ cages of ۵ numbers, were injected with ۱۰۰ μ l in the first cage, ۲۰۰ μ l in the second cage and ۴۰۰ l in the third cage, respectively, weekly for the treatment group during induction and for ۴ days for the post-induction group. Ovarian tissue sampling was performed ۴ days after the last injection and aromatase gene expression was assessed using Real Time-PCR technique. PCOS rats showed decreased aromatase gene expression, increased androgen-to-estrogen ratio, and increased body weight. It seems that the expression of aromatase gene in PCOS mice increased after injection of Escitalopram and we saw weight loss in treated rats. By binding LH to Leydig cells, Escitalopram increases or synthesizes testosterone and provides androgen as a substrate for aromatase. We see that it reduces the ratio of androgen to estrogen.

کلیدواژه ها:

Escitalopram ، Aromatase ، PCOS ، Estradiol valerate ، Steroidogenesis