The effect of Dog-rose (Rosa canina L) extract on in vitro maturation of immature oocytes in mice

سال انتشار: 1399
محل انتشار: بیست و یکمین کنگره ملی و نهمین کنگره بین المللی زیست شناسی ایران
کد COI اختصاصی: BIOCONF21_0645
زبان مقاله: انگلیسی
تعداد مشاهده: 299

نویسندگان

Assistant Professor, Department of Biological Sciences and Technologies, Islamic Azad University, Sari Branch, Sari, Iran

Ali Ahmadi

Student, Department of Biological Sciences and Technologies Islamic Azad University, Sari Branch, Sari, Iran

چکیده

Dog-rose plant with the scientific name of Rosa canina has a fruit that has been considered by the pharmaceutical industry due to its antioxidant properties. The aim of this study was to investigate the effect of Dog-rose fruit extract on in vitro maturation of immature oocytes of NMRI mice. In this study, animals were randomly divided into five groups: control, sham, experimental ۱, ۲ and ۳. Mice in the control group received only water and food for mice, and mice in the sham group received ۵۰۰ μl of distilled water for ۲۱ days. It was injected intraperitoneally and ۱۰۰, ۲۰۰ and ۳۰۰ mg / kg body weight of the extract was injected intraperitoneally to mice in the experimental group for ۲۱ days. They were incubated for ۲۴ hours. Oocytes that reached metaphase stage ۲ were then fertilized with sperm and after ۲۴ and ۴۸ results were evaluated. After injection of Dog-rose fruit extract, the rate of maturity in the eggs of experimental groups increased compared to the control group and the formation of ۴-cell embryos in groups ۱ and ۲ increased compared to the control group (p< ۰.۰۵). The results show that Dog-rose fruit extract due to its antioxidant compounds can be effective on the maturation of immature mouse eggs and the development of the resulting embryos.

کلیدواژه ها

Mice, in vitro fertilization of oocytes

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