Effects of ethanolic extract of Zataria multiflora and closantel on Fasciola hepatica egg hatching in in vitro conditions

Closantel is an antiparasitc drug widely used for treatment of fascioliasis as well as other parasitic infection like haemonchosis in cow, sheep and goat. The effects of Zataria multiflora in treatment of bacterial, parasite and fungal infection has been proved by several researchers. The aim of this study was to evaluate ethanolic extract of Zataria multiflora and closantel on hatching egg of Fasciola hepatica in in vitro condition. Zataria extract perform with ۷۰° ethanol and ۱۰۰, ۷۵, ۵۰, ۲۵, ۱۰, ۵, ۲, ۱ mg/ml dilution were prepared. The same dilution of closantel also prepared. For evaluation ۹۰۰ μl of Zataria multiflora extract or drug adjacent with ۱۰۰ μl egg extract for ۱۶ days in ۲۴°c. Results showed that in comparison with control group, closantel and Zataria multiflora extract were significantly decreased the egg hatching (P<۰.۰۵). These result in correlation with extract or drug concentration. Egg hatching in closantel groups were significantly decrease in compare with Zataria multiflora extract groups (P<۰.۰۵). In addition to, closantel effectively decrease the egg hatching percentage of Fasciola hepatica and it can be used as a first choice drug for treatment of infection. However, the Zataria multiflora extract show a significant decrease in egg hatching percentage and it seems the side effect of extract is less than closantel and it can be used as an alternative treatment protocol in Fasciola hepatica infection.

کلیدواژه ها:

Medicinal plants ، trematodes ، Sheep liver worm ، anti-parasitic