Looking at the effect of high fat diet on non-alcoholic fatty liver in mice using Glass’d effect size: A strong positive relation

Due to the importance of life style especially the diet on functional integrity of liver, we study the effect of high fat diet on the hepatocyte function and structure in mice. Consequently, we divided the mice into two groups; the first group, n=۸, fed with standard rodent chow, ۱۰Kcal, and the other group, n=۱۶, fed with high fat diet, standard chow plus ۴% cholesterol and ۵۰% corn oil. The mice kept for eight weeks and later their liver, and serum isolated and analyzed. We used the hematoxylin and eosin to dye the liver tissues and test the ballooning, inflammation, steatosis in the tissue. Also, we checked the fluctuation of liver enzymes such aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The result revealed in addition to the increase in the liver enzymes such as ALT and AST, inflammatory cells, ballooning, and macro-vesicles increased sharply in hepatocytes. Using Glass’d analysis for effect size, demonstrated there is strong positive relation between the high fat diet and the factors we estimated above. In contrast to the meaningfulness of the data, the confidence interval of the effect size demonstrate inconclusiveness of the result. Thus, we think the experiment must be repeated with bigger sample size to confirm the results.

کلیدواژه ها:

Liver ، Aspartate aminotransferase (AST) ، Alanine aminotransferase ، Inflammation ، Steatosis ، Ballooning