The effects of low fructose diet combined with α-lipoic acid supplementation on rat model of non-alcoholic fatty liver disease

Non-alcoholic fatty liver disease (NAFLD) is one of the most common liver diseases which could lead to fibrosis and cirrhosis .Insulin resistance often associat with NAFLD and progression of it. Previous studies have reported that fructose and lipoic acid could prevent insulin resistance. In this study, a low-fructose diet combined with α-lipoic acid was used to evaluate its effect on hepatic steatosis, insulin resistance and some parameters of NAFLD due to high fat diet in the Sprague-Dawley rats. Male rats were divided into five group (n=۸). Normal control group (NC), high fat diet group (HF), fructose group (Fru), lipoic acid group (Lip) and fructose plus lipoic acid group (Fru+Lip). The high fat control group was orally treated with the high fat emulsion diet (HFD) and fructose group orally treated with the HFD plus fructose (۱g/kg), Lipoic acid group orally treated with the HFD plus lipoic acid (۶۰g/kg) and fructose plus lipoic acid group orally treated with the HFD plus fructose (۱g/kg) combined with lipoic acid (۶۰g/kg) once per day via gavage for six weeks. After six weeks, the rats were sacrificed. Serum was collected for measurement of biochemical and ELISA analyses and also liver for hepatic biochemical parameters. Adipose tissue was collected for measurement of PGC-۱α expression by Real time PCR. After six weeks, the plasma level of lipid profile, insulin resistance, TNF-α, hepatic content of malondialdehyde (MDA), and triglyceride (TG) increased and PGC-۱α expression decreased in HFD group. In fructose group, serum glucose, insulin resistance, serum and liver lipid profile, TNF-α, and hepatic malondialdehyde significantly decreased and PGC-۱α expression increased compared to the high fat control group (P<۰/۰۵). In addition, in Fructose plus Lipic acid group, serum glucose, insulin resistance (HOMA-IR) significantly decreased compared to the fructose group (P<۰/۰۱). This study showed that a low-fructose diet could prevent insulin resistance and hepatic steatosis that they are features of nonalcoholic fatty liver disease (NAFLD). These data also indicate that lipoic acid improved this effect and low fructose diet combined with α-lipoic acid supplementation might provide a beneficial treatment for NAFLD.

کلیدواژه ها:

Glucose ، Insulin resistance ، High fat diet ، Fatty liver disease