Sample size optimization of Anodonta cygnea (Linnaeus, ۱۷۵۸) in ecotoxicological studies through the morphological features

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 89

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شناسه ملی سند علمی:

BIOCONF21_0576

تاریخ نمایه سازی: 7 شهریور 1400

چکیده مقاله:

The use of bivalves as the sessile benthos is very ordinary in aquatic toxicological monitoring studies. On the other hand, the bioethical considerations compel us to take minimum samples from the environment as much as possible without reducing the trial quality. Therefore, we experimentally assessed the correlation between morphometric characteristics of Anodonta cygnea and its acetylcholinesterase (AChE) activity inhibited by diazinon. For this purpose, the AChE activity of A. cygnea was examined after exposure to various concentrations of diazinon for ۲۱ days by Elman's method using an ELISA microplate reader. Some principal morphometric indices such as total length (TL), width, height, length of the hing, weight of soft parts, weight of valves, inlet, and outlet size were measured through standard instructions. Results showed that the average level of the enzyme activity in the adductive muscle was ۲۷.۲۸۵ nmol/min/mg protein. There was a significant correlation between TL and AChE inhibition. Thus, the maximum inhibitory was observed in the size class ۸۵۰-۹۵۰ mm. Moreover, an increase in inlet size leads to more intake and following that, more inhibition. In conclusion, using these findings in the sampling procedure can enhance the selected and most relevant samples in the field studies.

نویسندگان

Nima Shiry

Iran Fisheries Organization, Administration of Khuzestan Province, Abadan, Iran

Negin Derakhshesh

Iran Fisheries Organization, Administration of Khuzestan Province, Abadan, Iran

Ahmad Rahbar

Iran Fisheries Organization, Administration of Khuzestan Province, Abadan, Iran

Seyed Jalil Alavinia

Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran