Potential Evaluation of Polycaprolactone nanofiber scaffolds for culturing human stem cells and differentiation to neural cells

The differentiation of human mesenchymal stem cells (hMSC) to neural-like cells on scaffolds has excellent potential for bionanomaterial-cell transplantation treatment of neurodegenerative diseases and the damaged nervous system. The multipotential features of hMSC.It is shown that the non-uniform distribution of equivalent stress on the longitudinal and transverse have been discovered in various tissue engineering researches. This investigation shows the in-vitro development and neural differentiation of hMSC in ۳D and ۲D environments. The ۳D environment which used in this study is nanofibrous polycaprolactone (PCL). We examined the differentiation capability of human bone marrow-derived from mesenchymal stem cells (MSCs) to neural cells on the random polycaprolactone (PCL) nanofibrous scaffolds, and on the tissue plate. Researches in this area have pointed to the achievement that in-vivo cells interact with the extracellular matrix, made of nanofibrous at sub-micron scale, which gives mechanical maintenance to the cells and plays a functional role in the control of cellular behaviour. Stem cells are developing as a fundamental tool in the evolution of tissue engineering and regenerative medication. PCL characterization was determined employing scanning electron microscopy (SEM), contact angle, and tensile instrument. The differentiation of MSC was conducted applying neural inducing agents, including retinoic acid, epidermal growth factor (EGF), fibroblast growth factor (FGF-۲), and Ibmx in DMDM/F۱۲. Scanning electron microscope (SEM) and optical microscope outcomes revealed neural morphology and reproduction of mesenchymal cells on PCL nanofibrous scaffolds. The differentiated mesenchymal cells on nanofibrous scaffolds express neural gene markers including; β- tubulin III and Map۲ on the day of ۱۴. Our investigation on the differentiation of hMSCs to neural cells on nanofibrous scaffolds recommends their potential use towards neural improvement. This study conducted in ۲۰۱۱.

کلیدواژه ها:

Polycaprolactone scaffolds ، Tissue engineering ، Human mesenchymal stem cells ، Neural cells ، Cell therapy.