Effect of Resveratrol on amnesia caused by complete sleep deprivation in male laboratory rats

Background: Sleep disorders and amnesia have been known for many years as one of the most important health problems. Sleep disturbance refers to cases in which a person complains of one or more of the problems such as amnesia, frequent awakenings during the night sleep, increased drowsiness during the day or abnormal movements, moods and feelings during sleep. Sleep deprivation impairs the perception of pain, forgetfulness, and depression. Approximately ۳۰ to ۴۰% of ordinary people suffer from it, this problem can have adverse effects on people's health and longevity.Purpose: This study was performed to evaluate the effect of Resveratrol on amnesia caused by complete sleep deprivation in male laboratory rats at Tehran Islamic Azad University of Medical Sciences during ۲۰۱۹-۲۰.Methods: In this animal study, ۹۶ male rats weighing ۲۲۰ to ۲۴۰ g, purchased from the Faculty of Pharmacology, University of Tehran, were randomly divided into ۳ main groups, including ۷-day control group, ۷-day sham and sleep deprivation. It was ۷ days (SD with Non-REM ۴۸h). Each of the main groups included ۴ subgroups of control, vehicle, and drug (۱۰۰ and ۲۰۰ mg / kg). The controls were normal mice receiving no medication. Vehicle received only ۰.۲۵% carboxymethylcellulose solution (Sigma Aldrich) with ۰.۹% saline or rapamycin intraperitoneally daily for ۷ days. The drug was given only Resveratrol daily for ۷ days at doses of ۱۰۰ and ۲۰۰ mg / kg intraperitoneally. Each animal was tested only once. The day after the last injection (ie the eighth day), train behavioral tests (fear condition and shock ۳ times), locomotive, hot plate, and forced swimming test were performed on all rats, then all rats were given ۴۸ hours of non-REM sleep deprivation and Again, these behavioral tests were taken from rats.Results: To evaluate conditioned fear memory, the rate of delay until the first freezing in the auditory stage was increased in mice that received the body (P <۰.۰۰۱), which means memory impairment. On the other hand, both doses of Resveratrol significantly reduced the delay until the first freezing (P <۰.۰۰۱), which means improved memory function. In addition, the rate of delay until the first freezing in the visual phase increased in mice that received the body (P <۰.۰۰۱), which means memory impairment. On the other hand, the dose of ۲۰۰ mg / kg of Resveratrol significantly reduced the delay until the first freezing (P <۰.۰۵), which means improved memory function. In the FST test, the rate of immobility increased in mice that received the body (P <۰.۰۰۱), which means an increase in depressive-like behavior. On the other hand, both doses of Resveratrol (۱۰۰ mg / kg: P <۰.۰۱ - ۲۰۰ mg / kg: P <۰.۰۰۱) reduced immobility, which means a reduction in depressive-like behavior. Based on the results of the hot plate test, there is a statistically significant difference between the data of the control groups (with normal sleep) (F۳.۲۸ = ۱۳.۰۲, P <۰.۰۰۱). Also, the pain threshold decreased in mice receiving naranginine at a dose of ۲۰۰ mg / kg (P <۰.۰۵).Conclusion: Our results show that sleep deprivation impairs memory function. Increased inflammation, increased apoptosis, suppressed neurogenesis, and decreased BDNF expression can cause depression or depressive-like behavior. Increased pain perception thresholds in sleep-deprived groups are probably due to increased adenosine levels following prolonged waking. However, the effect of sleep deprivation or restriction on pain is contradictory. Also, no mechanism is suggested to reduce the pain perception threshold following Resveratrol injection and more research is needed in the future. Locomotor activity could also affect the performance of mice in the forced swimming test, although it could not affect memory performance.

کلیدواژه ها:

Resveratrol ، amnesia ، memory ، pain ، depression ، complete sleep deprivation ، male rats