A Comparison of phylogenetic groups between mastitis causing strains of Escherichia coli and fecal isolates
محل انتشار: بیست و یکمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 244
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شناسه ملی سند علمی:
MEDISM21_352
تاریخ نمایه سازی: 23 مرداد 1400
چکیده مقاله:
Background and Aim : Worldwide phylogenetic analyses have demonstrated that virulent extra intestinal pathogenic Escherichia coli (ExPEC) strains belong mainly to group B۲ and, to a lesser extent, to group D. On the contrary, most of the commensal strains are associated with group A or group B۱. The most important members of ExPEC group are avian pathogenic E. coli (APEC), Uropathogenic E. coli (UPEC), and Mammary pathogenic E. coli (MPEC) which are responsible for extra intestinal diseases in animals or humans. This study tries to elucidate the status of mastitis causing E. coli strains as a member of ExPEC group and their relation to commensal E. coli isolates in terms of their phylogenetic groups.Methods : Methods: A total of ۶۴ Escherichia coli isolates (mastitis causing isolates= ۳۲, fecal isolates from the same cows= ۳۲) were examined for determination of phylogroups using quadruplex PCR described by Clermont et al., ۲۰۱۳.Results : Results: Most of the mastitis causing isolates belonged to the phylogroups A (۴۳.۷۵%) and B۱ (۴۰.۶۲%), while ۶۲.۵% of fecal isolates were categorized as B۱. The group C had the least members of both mastitis causing isolates (۶.۲۵%) and fecal isolates (۳.۱۲%). No members of the phylogroups D and E were detected among mastitis causing isolates and fecal isolates, respectively. Furthermore, among ۳۲ paired samples, the phylogenetic groups of ۱۲ paired samples (۱۲ milk and ۱۲ fecal samples from the same cows) were the same.Conclusion : Conclusion: The high prevalence of MPEC members in groups A and B۱ may represent the commensal nature of these isolates.
کلیدواژه ها:
نویسندگان
Gholamreza HashemiTabar
Hamideh Kalateh Rahmani, Gholamreza Hashemi Tabar, Mahdi Askari Badouei, Babak Khoramian Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Department of Clinical Sciences, Faculty of Veterinary Medi
Hamideh Kalateh Rahmani
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Mahdi Askari Badouei
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Babak Khoramian
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran