Antibiotic resistance and prevalence of imp-۲,vim-۲, sim and ndm genes in Escherichia coli strains isolated of cause urinary tract infections

Background and Aim : Urinary tract infection (UTI) is the second most common human infection, especially among women, caused by the genus of Enterobacteriaceae family, especially Escherichia coli. Metallobalactamase (MBL) with ability to hydrolyze beta-lactam antibiotics especially carbapenems, are one of the main enzymes that cause antibiotic resistance. The aim of the present study is evaluation of the antibiotic resistance and the frequency of imp-۲, vim-۲, sim and ndm genes among E. coli strains isolated of urinary tract infections in Ahvaz city.Methods : The two hundred samples collected from people with UTI were examined after culture and isolation. The resistance pattern related to ampicillin, kanamycin, cefotaxime, streptomycin, ciprofloxacin, imipenem and meropenem was investigated according to CLSI ۲۰۱۸. To determine MBL-producing isolates, imipenem and meropenem resistant strains were evaluated by PCR method for the presence of imp-۲, vim-۲, sim and ndm genes.Results : The highest resistance was observed against ampicillin (۸۷.۵%). The percent of resistance related to kanamycin, cefotaxime, streptomycin, ciprofloxacin, imipenem and meropenem was as follow: ۲۸%, ۶۳%, ۴۶%, ۵۰%, ۴.۵% and ۵% respectively. The gene of ndm was found in ۸ isolates; while none of isolates harbored imp-۲, vim-۲ and sim genes.Conclusion : The detection of ndm gene in these isolates indicates the spread of this type of resistance among pathogenic isolates. MBL resistance leads to the ineffectiveness of the last line of antibiotics. Therefore, it is need countermeasures against this type of antibiotic resistance.

کلیدواژه ها:

Escherichia coli ، UTI ، antibiotic resistance ، MBL ، disc diffusion method