Evaluation of Cytomegalovirus in leukemia by PCR molecular method

Background and Aim : Introduction: Leukemia begins with the destruction of hematopoietic bone marrow cells and with the turmoil in the blood appears (Immature blood cell proliferation, homeostasis disorder, degrees of inflammation, mutation, immunodeficiency, antioxidant deficiency and infectious agents), Due to its high numbers in Iran, we decided to measure in Leukemia by PCR method, to quantify the prevalence of cytomegalovirus (CMV) as the largest member of the herpes family that can cause latent infections for a lifetime,Methods : Methods: ۱۰۰ leukemic blood samples and ۱۰۰ healthy blood samples were collected from Tehran hospitals and DNA extraction was performed by phenol-chloroform method. CMV PCR test was optimized and evaluated for specificity and limit of detection (LOD). Optimized test was performed on all patient and healthy samples along with positive and negative controls. The PCR product was identified by agarose gel electrophoresisResults : Results: PCR test was optimized, and ۲۵۷ bp product was observed in agarose gel electrophoresis. In specificity test, No product other than the CMV sample was observed. LOD was ۱۰۰ Copy/reaction, and all healthy controls were negative and ۳۳ of the samples were CMV positive.Conclusion : Conclusions: Cancer has many causes, including leukemia. The role of infectious agents in cancers is increasing every day. This study shows that CMV can be considered as an infectious and influencing factor in leukemia.

کلیدواژه ها:

Detection ، leukemia ، Cytomegalovirus ، infections ، PCR