Identification of Antibacterial, Antifungal and Anticancer Activity of Five Strains of Soil Microorganisms Isolated From Tangkuban Perahu Mountain by Fermentation

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 172

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شناسه ملی سند علمی:

MEDISM21_291

تاریخ نمایه سازی: 23 مرداد 1400

چکیده مقاله:

Background and Aim : Soil microorganisms from the group of actinomycetes are well accepted to be the best producer of antibiotics. microorganisms were isolated from soil taken from Tangkuban Perahu mountain.The aim of this study was to screen the highest antimicrobial and anticancer activity of strains isolated from Tangkuban Perahu mountain.Methods : One gram of dried soil sample was taken in ۱۰۰ mL of sterile distilled water and mixed thoroughly in a shaker for ۱۵ minutes at ۱۲۰ rpm. Five strains were investigated in this study designated TP۱, TP۲, TP۳, TP۴, and TP۵, respectively. Morphological, biochemical and molecular identifications were conducted for all five strains.Results : These isolates were shown to be closely related to Nocardia sp. YIM ۶۵۶۳۰ (۹۰%), Streptomyces galbus (۹۹%), Aspergillus unguis (۸۶%), Paecilomyces marquandii (۱۰۰%) and Nocardia niigatensis (۹۵%), respectively. Production of antibacterial, antifungal and anticancer metabolites was done by fermentation. Screening for bioactivity of five isolates was done by testing the fermentation broth against resistant and pathogenic bacteria, fungi and T۴۷D breast cancer cell line. TP۲ strain showed the best bioactivity; the metabolite was purified by extraction with ethyl acetate. Antibacterial, antifungal and anticancer activities from the ethyl acetate extract of TP۲ strain were tested by agar diffusion, microdilution and MTT.Conclusion : The extract was shown to be active against methicillin resistant Staphylococcus, methicillin sensitive Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus, vancomycin resistant Enterococcus, Escherichia coli, Microsforum gypseum with the minimum inhibitory concentration (mg/mL) and diameter of inhibition (mm): ۱۵۰, ۳۵; ۱۵۰, ۳۰; ۳۰۰, ۳۵; ۳۰۰, ۳۵; ۳۰۰, ۲۹; ۴.۷, ۳۶, respectively. The IC۵۰ value of the T۴۷D cell line was ۴۵۷ mg/mL.

کلیدواژه ها:

antibacterial ، antifungal and anticancer activities ، ethyl acetate extraction ، fermentation ، identification

نویسندگان

Melika Farzin

Msc technical staff in Medical Sience of Shahid Beheshti University