Parisa Abedi Elkhichi - Medical Microbioogy Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
Sama Reza Soltani - Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Hossein Dabiri - Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Ehsan Nazemalhosseini Mojarad - Gastroenterology and Liver Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abbas Yadegar - Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Background and Aim : Colorectal is the cancer of the colon or rectum, and its incidence is steadily rising in developing nations(۱). One of the risk factors is infectious and microbial agents such as Campylobacter spp.. Campylobacter is caused many undiagnosed cases of diarrhea in developing countries, including Iran(۲, ۳). Recent studies have shown a relation between Campylobacter spp. infection with the risk of colon cancer(۴). Due to the high prevalence of this bacterium in developing countries such as Iran and its role in infection and gastrointestinal cancer and its lack of diagnosis in clinical laboratories, in this article, we investigated the prevalence of this bacterium in colorectal cancer.Methods : This study was performed on tissue samples from patients suspected of colorectal cancer referring to Gastroenterology and Liver Disease Research institutes in Taleghani Hospital from ۲۰۱۷ to ۲۰۱۸. In the next step, DNA was extracted by the available kit(QIAamp DNA Mini). The presence of DNA in samples was confirmed by using Nano drop. Then the presence of Campylobacter spp. was determined by polymerase chain reaction (PCR) using specific primers targeting ۱۶srRNA gene and PCR products were run on ۱.۵% gel electrophoresis and results were observed using gel doc. finally, the data was analyzed by SPSS ۱۶.۰ statisticalResults : A total of ۸۶ patients that ۱۸ controlled and ۶۸ were cancer patients were studied. This study included ۳۲.۶% females and ۶۲.۸% males (Mean age ۵۶.۴ years; age range ۲۱ to ۸۷ years). In the patient group the areas of involvement were respectively Rectum ۲۲ case (۳۱%), Recto sigmoid ۱۲ cases (۱۷%), Colon ۱۲ cases (۱۷%), Descending colon ۹ cases (۱۵%), And other areas related to Cecum, Duodenal, Rectal, Transverse colon, Ascending shown in Figure ۱. Of ۸۶ biopsy specimens, ۱۴(۲۰%) were positive for Campylobacter spp. (Figure ۲) .Conclusion : Campylobacter spp. produces toxins one of them is a cytolethal distending toxin (Cdt). This toxin causes DNA damage and secretion pro-inflammatory cytokines. Which promotes tumor progression and cancer through angiogenesis and metastasis. Thus, based on our previous studies and the achievement of our paper, Campylobacter can play a role in colon cancer.

Campylobacter spp., colorectal cancer