Phenotypic and genotypic characterization of macrolide resistance among Staphylococcus aureus isolates obtained from foodstuffs products

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 192

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شناسه ملی سند علمی:

MEDISM21_266

تاریخ نمایه سازی: 23 مرداد 1400

چکیده مقاله:

Background and Aim : Currently, frequently use of macrolide antibiotics for serious staphylococcal infections has led to the emergence of Staphylococcus aureus resistant to MLSB antibiotics. There were some information about the type of MLSB resistance in a healthcare setting, however, no sufficient data has been founded on MLSB prevalence among S. aureus isolated from food sources, Therefore, the aim of this was determine the prevalence of macrolide resistance among S. aureus isolates collected from various foodstuffs products.Methods : A total of ۸۴ bacterial samples was collected from several foodstuffs products in the city of Isfahan. Initial identification was performed based on the phenotypic and genotypic test. Double-disk diffusion tests (D-test) were performed based on CLSI guideline. In general, DNA of all isolates was extracted using boiling method. Moreover, to detect of mecA, ermA, ermB, and ermC, PCR was performed.Results : Totally, ۸۴ bacterial samples were collected from several foodstuffs products. Among them, ۲۱ isolates were S. aureus (۲۵%). Furthermore, based on antimicrobial tests, all of the S. aureus isolates (۱۰۰%) were susceptible to clindamycin; whereas ۲۰ isolates were susceptible to erythromycin (۹۵.۲۴%) and just ۱ (۴.۷۶%) isolate was resistant phenotype to iMLSB (resistant to erythromycin and susceptible to clindamycin). Molecular analysis revealed that two (۹.۵۲%) were MRSA (mecA positive) and ۱۹ (۹۰.۴۸%) were methicillin-susceptible S. aureus (MSSA). In addition, ermC, ermA and ermB gene were detected in ۲۳.۸%, ۱۴.۲۸% and ۹.۵% isolates. Two isolates harbor ermA and ermC genes and one isolates carried ermC and ermB, simustansly. Moreover, it should be noted that some isolates did not have any erm genes.Conclusion : In conclusion, our results indicated that erythromycin-resistant and ermC were the predominant phenotypes and genes. The results revealed that the various mechanisms of erythromycin resistance are expanding among S. aureus isolates collected from several foodstuffs products.

نویسندگان

Laleh Hoveida

Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Mehrdad Halaji

Department of Microbiology, School of medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Parisa Zeraati

Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran