Evaluation of the frequency of Escherichia coli pathogroups in Brassica oleracea cultivars

Background and Aim : Pathogenic Diseases resulting from food with microbial contamination have widely distributed in many parts of the world. Diarrhea, for example, is one of the major causes of death of children in developing countries, with approximately ۲ million deaths annually. Escherichia coli is one of The most important of foodborne pathogenic bacteria . The aim of the current study was to determine the frequency of diarrheagenic E. coli pathotypes such as Enteropathogenic E. coli (EPEC), Enterotoxigenic E. coli (ETEC), Enteroaggregative E. coli (EAEC) and Shiga toxin producing E. coli in Brasica oleracea cultivars in order to provide information on the assessment of diarrheagenic E. coli pathogenesis risk in this group of vegetables.Methods : For this purpose, ۱۰۰ samples of cabbage were collected in Tehran, including kale, cauliflower, broccoli, and brussels. Pathotypes were identified by PCR molecular method using specific primers of virulent genes eae, bfpA, lt, st, pCVD۴۳۲, stx۱ and stx۲.Results : The results showed that the prevalence of diarrheagenic E. coli strains was ۷% and the prevalence of EPEC was ۳%, with two isolates from leaf cabbage and one isolate from cauliflower. All EPEC isolates were atypical. The ETEC frequency was ۳%, with one isolate from cabbage leaf, one isolate from cauliflower, and one isolate from broccoli.Conclusion : The results of the present study revealed the possible role of this group of vegetables as a source of contamination with E. coli strains causing diarrhea.

کلیدواژه ها:

diarrheagenic Escherichia coli ، PCR ، raw vegetables ، Brassica oleracea