survey on carrier state of leptospirosis in dogs

Background and Aim : Leptospirosis is an infection caused by spirochetes of the Leptospira genus that are the most common zoonosis worldwide and is endemic in tropical environments. Clinically recovered dogs frequently become asymptomatic renal carriers, and as such can be an important source of human leptospirosis. leptospirosis is now recognized as an important re-emerging disease in dogs. in this research was decided to study the incidence of leptospirosis carrier state in vaccinated and unvaccinated dogs.Methods : For this purpose, ۱۵۰ urine samples were collected from two group (urban or rural) of dogs in Ahvaz city. ۵ ml of urines were centrifuged, and concentrated to ۰.۵ ml. Darting motility of leptospira in urine was studied by wet mount technique from concentrated urine and dark field microscope. After DNA extraction by commercial kit (RahaZist Padtan-IRAN), contamination of each sample to Leptospira genus and ۴ its serogroup (Icterohaemorrhagiae, Pomona, Hardjo and Grippotyphosa) were evaluated by PCR and PCR-RFLP technique, respectively.Results : Based on results, regardless of confirmation of leptospira genus contamination in ۷۹ (۵۰.۶۷%) samples by PCR, in none of them darting motility observed. Restriction Fragment length polymorphism by HindIII و HeaIII enzymes, defined that abundant serogroup was Icterohaemorrhagiae (۵۱.۸۵%), and thereafter Pomona and Hardjo (۲۴.۰۷%). In this research not important relation between leptospira contamination with age, breed, gender, vaccination, urban or rural was observed by Chi-squared test.Conclusion : At all, Contamination in dogs more than ۲ years old, male, Shih-tzu terrier, unvaccinated and rural, was more than dogs with less than ۲ years old, female, vaccinated and urban with another breed and Icterohaemorrhagiae was predominant serotype.

کلیدواژه ها:

Dog ، Leptospirosis ، PCR-RFLP