Invitro study of human amniotic fluid in the control of Impetigo common pathogen

Background and Aim : Impetigo is an infective dermatitis and a common skin disorder. It can be very difficult to control in some cases and might be life-threatening if not treated, especially in children. The aim of this study was to investigate the antibacterial effect of amniotic fluid of pregnant women on drug-resistant Staphylococcus aureus isolates from children with impetigo.Methods : S. aureus isolates were collected from children suspected of having impetigo. The isolates were identified using microbiological and biochemical tests. Antibiotic susceptibility was evaluated using the Kirby-Bauer method according to the M۱۰۰-S۲۵ (۲۰۱۵) guidelines. The antibacterial effect of different concentrations of amniotic fluid on drug-resistant S. aureus isolates was investigated by the disk diffusion method.Results : Of ۳۵% S. aureus isolates, ۸۸% were sensitive to teicoplanin and vancomycin, while ۶۵% of the isolates were resistant to penicillin, which is conventionally used for treatment of impetigo. Based on the results, ۶۴% of drug-resistant isolates were sensitive to amniotic fluid. There was a significant relationship between the concentration of amniotic fluid and the diameter of bacterial growth inhibition zone (P<۰.۰۱).Conclusion : The amniotic fluid of pregnant women has favorable antibiotic effects against antibiotic-resistant S. aureus isolates from children with impetigo. Therefore, it can be potentially used for natural biocontrol of this common infection.

کلیدواژه ها:

Amniotic fluid ، impetigo ، Drug resistance ، Staphylococcus aureus