Comparison of the effect of liposomes of red pepper extracts (Capsicum frutescens) and Allium cepa onion against Staphylococcus aureus with beta-lactamase gene isolated from packaged minced meat in Yazd

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 226

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شناسه ملی سند علمی:

MEDISM21_195

تاریخ نمایه سازی: 23 مرداد 1400

چکیده مقاله:

Background and Aim : Background: Staphylococcus aureus is one of the most important pathogenic microorganisms in meat products, especially those that come into contact with the hand during repeated production. Beta-lactamase drugs, especially the new generation of Cephalosporins, is used to treat most infections caused by Staphylococcus aureus, but the production of beta-lactamase by some strains has failed to treat infections associated with the organism. Objective: The aim of this study was to evaluate the degree of contamination and compare the antimicrobial effect of liposomes of red pepper extracts and red onion on Staphylococcus aureus with beta-lactamase gene isolated from minced meat in Yazd.Methods : Methods: For this purpose, sampling of ۳۵ packaged meat distribution and supply centers in Yazd city was performed under hygienic conditions and all samples were tested for the presence of Staphylococcus aureus with beta-lactamase gene tested by biochemical methods and molecular confirmation with PCR test. Were placed. The antibacterial effect of the liposomes of red pepper extracts and red onion on the mentioned isolates was evaluated with the tests of minimum inhibitory effect (MIC), minimum lethal effect (MBC), well release and bacterial growth curve.Results : Results: The results showed that ۱۸% of the samples were infected with Staphylococcus aureus bacteria with the beta-lactamase gene. The liposomes of red pepper extracts and red onion showed a good antibacterial effect against these isolates, and in all tests, the liposomes of red pepper extract was more effective than green onion.Conclusion : Conclusion: By proving the stronger antimicrobial effect of red pepper extract liposomes, it is recommended that in dishes such as grilled types of meat prepared from minced meat, be sure to use the liposomes extract system in addition to onions.

نویسندگان

Mohammad Javad Forouzani-Moghaddam

Department of food microbiology, School of Paramedical, Iran University of Medical Sciences, Tehran, Iran

Nazanin Zare

Department of food Biology, School of Science, Shahid Bahonar University, Kerman, Iran