Protective effects of egg yolk immunoglobulins (IgYs) against recombinant immunogens Ctxb, OmpW and TcpA on infant mice infected with Vibrio cholerae

Background and Aim : Vibrio Cholerae causes Cholera and other infections, especially in children under five years of age. Cholera toxin (CT), toxin-coregulated pilus (TCP) and outer membrane protein W (OmpW) are three major virulence factor of this bacterium. The emergence of antimicrobial-resistant (AMR) strains and the absence of a comprehensive and flawless vaccine, has prompted other treatments. So in this study, IgYs against recombinant proteins Ctxb (responsible for the CT binding to eukaryotic cells), TcpA (enhances bacterial attachment to enterocytes) and OmpW, in single, coupled or combined forms, were produced and their protectivity were evaluatedMethods : IgYs titer were analyzed by protein and whole cell ELISA and their neutralizing effects on CT and heat-labile enterotoxin (LT) of Escherichia Coli were evaluated in Y۱ cell culture. IgY titers were gavage administered to different groups of infant mice infected with V. cholerae.Results : Based on the results, Anti-CtxB IgY showed the highest titer and had the most neutralization effects on the toxins, while the most protectivity of the infant mice were obtained by Anti-TcpA IgY. No considerable difference was observed in protectivity or antibodies titer produced against single or combined proteins.Conclusion : The results of ELISA, cell culture, and animal challenge indicated a higher antibody titer and a higher protection achieved using the IgYs based on single antigen. Among them Anti-TcpA IgY selected as the best antibody due to more protection in infant mice model.

کلیدواژه ها:

Vibrio Cholerae ، Cholera ، IgY ، CtxB ، TcpA ، OmpW