Phage therapy; renewed method for multi drug resistant Staphylococcus aureus

Background and Aim : Introduction and objective: Bacteriophages are group of viruses that infect and replicate inside host bacteria and finally lyse the host cells. Phages require a specific receptor for penetrating to the host bacterial cells. The therapeutic use of phage to treat bacterial infections (pahge therapy) was conceived by Felix d'Herelee nearly a century ago. Due to the discovery of antibiotics that provide greater breadth and potency, phage therapy lagged behind. The widespread resistance to antibiotics push microbiologists to find alternative therapeutic methods to replace with common methods for therapy of bacterial infection, phage therapy is used again and is known as an effective method.Methods : Method : in this study ۱۰۳ samples of Staphylococcus aureus were isolated from Isfahan hospital, Iran. The resistance to methicillin, clindamycin, erythromycin, cefoxitin, tetracycline, ciprofloxacin and gentamycin were measured and determined by disk diffusion method. Staphylophages were isolated from urban swage then purification of it. To indicate the effect of Staphylophages was used double layer agar method.Results : Result: out of ۱۰۳ samples, ۳۳% were clindamycin resistant, ۸۵% were methicillin resistant, ۳۱.۵% were erythromycin resistant, ۴۶.۶% were cefoxitin resistant, ۴۳.۶% were tetracyclin resistant, ۵۱.۱% were ciprofloxacin resisrant and ۲۵.۵% were gentamycin resistant. Fifty samples were formed plaque which indicated they were lysed by isolated staphylophage.Conclusion : Conclusion: anti bacterial drug resistante is important problem, to deal with this problem ,alternative method must be replaced. In this study, staphylophages had lytic effect on Staphylococcus aureus multi drug resistance. Phage therapy could be effective method that replaced with chemical tehrapy.

کلیدواژه ها:

phage therapy ، bacteriophage ، staphylococcus aureus ، antibiotical resistant