In vitro activity of Bacillus subtilis metabolites in the control of mucosal candidiasis in the elderly

Background and Aim : Bacillus species as antagonistic bacteria are promising biocontrol agents of infections. This study aimed to investigate the antifungal activity of Bacillus subtilis metabolites on the growth of clinical strains of Candida isolated from the elderly.Methods : ۸۲ elderly patients suspected of oral candidiasis were studied. Candida species were identified and isolated by culture on CHROM agar Candida medium and API ۲۰CAUX system. In order to isolate B. subtilis from ۱۰ soil samples of Gorgan (North of Iran), routine and specific laboratory tests were used. Antifungal activity of B. subtilis metabolites on Candida isolates was investigated by agar diffusion method.Results : ۷۵.۶% of the isolates were confirmed as Candida strains of three species of albicans, tropicalis and glabrata, with the highest abundance (۷۷%) belonged to C. albicans. Bacterial metabolites had the highest inhibitory effect (۶۶.۷%) on C. glabrata (with an inhibition zone diameter of ۱۹.۱mm) and the least effect (۱۶.۷%) on C. albicans (P<۰.۰۱).Conclusion : Native isolates of B. subtilis have effective anti-Candida effects, so using pure extracts of these isolates to control candidiasis may be effective in the future.

کلیدواژه ها:

Candida ، Bacillus subtilis ، Antagonism