Investigation of tetracycline resistance genes in Escherichia coli isolates from Patients with Urinary Tract Infection; Yasuj City, Southwest Iran

Background and Aim : Urinary tract infections (UTIs) are the most disregarded diseases in both developing and developed countries and accountable for one fourth of the health care related infections. Microbial resistance to antibiotics due to treatment of bacterial pathogens and extended use of different antibiotics is an increasing public health problem worldwide. The aim of this study was to determine the prevalence of tetracycline resistance genes (tetA and tetB) in Escherichia coli isolates that cause urinary tract infections.Methods : Totally, ۱۲۹ strains of Escherichia coli isolated from UTIs patients, using bacterial culture method. Isolates were tested for susceptibility to ۱۰ antimicrobial drugs by using disk diffusion method and the presence and identification of tetA and tetB genes were determined by Polymerase Chain Reaction (PCR). Data were statistically analyzed using SPSS v.۱۸ (SPSS Inc.,Chicago, IL, USA).Results : Among tetracycline-resistant isolates, ۷۷ (۵۹.۷%) isolates with tetA and ۸۶ (۶۶.۷%) isolates with tet B gene and ۶۱ isolates with both genes were identified.There was a statistically significant association between tet A gene and resistance tetracycline (P = ۰.۰۰۶) and tet B gene and resistance tetracycline (P = ۰.۰۳۷).Conclusion : The results of this study showed that the percentage of tetracycline resistance genes is high in patients with urinary tract infections. This can indicate an overdose of antibiotics. Therefore, due to the prevalence of urinary tract infections with Escherichia coli in the community and the spread of resistance and pathogenic factors, it is necessary rapid and accurate identification of this bacterium and its resistance factors to treat such infections.

کلیدواژه ها:

Antibiotic Resistance ، tetracycline ، tetA and tetB ، Escherichia coli ، Urinary Tract Infection ، Uropathogenic