The morphological analysis by bright field and transmission electron microscopy of Escherichia coli producing ESBL with a Hypericum perforatum aqueous extract

Background and Aim : The increasing resistance of microorganisms to conventional chemicals and drugs is a serious and evident worldwide problem that has prompted research into the identification of new biocides with broad activity. Plants and their derivatives are often used in folk medicine. aqueous extract and their components have activity against a variety of targets, particularly the membrane and cytoplasm, and in some cases, they completely change the morphology of the cells. The aim of study was to investigate the effect of aqueous Hypericum perforatum extract on the morphological properties of Escherichia coli producing ESBLs (extended-spectrum beta-lactamases).Methods : ۴۰ strains of Escherichia coli ESBLs from clinical purified samples were taken from Arak medical university microbial research center infectious diseases and E. coli ATCC۲۵۹۲۲. Antibiogram and Biochemical tests were taken in order to verify Escherichia coli. The Hypericum perforatum plant is prepared freshly from farm herb. Before drying plant in a traditional way, it should be washed with water for several times, Hypericum perforatum aqueous extracts were excavated by means of reflux device with distillation and investigating its morphological investigation, taken by the use of electronic microscope and light microscope.Results : Obtained result showed that aqueous extract of Hypericum perforatum leading to an increased in coccobacilli status, increased degradation and changes in bacterial pigmentation properties.Conclusion : The action of aqueous extract and their components on bacteria remains a focal area for future research.

کلیدواژه ها:

Hypericum perforatum ، E. coli ، Microbial morphology ، Drug resistance