Antibacterial effects of alcoholic extracts of Caracrol, thyme and Ferulago
محل انتشار: بیست و یکمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 348
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شناسه ملی سند علمی:
MEDISM21_086
تاریخ نمایه سازی: 23 مرداد 1400
چکیده مقاله:
Background and Aim : Background and purpose: Antibiotic resistance of bacteria is one of the most common problems in medical sciences. In this study, the antibacterial effect of alcoholic extract of caracol, thyme and ferulago plants on four bacteria of Escherichia coli pathogen, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus agalactia was examined under laboratory conditionsMethods : Materials and Methods: In this experimental study, the alcoholic extract of three plants, caracrol, thyme and ferulago, was used. The total extract of the mentioned plants in different concentrations (۱۰۰ mg / ml, ۲۰۰, ۴۰۰, ۸۰۰) was affected by the method of propagation in agar and tube dilution on Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus agalactia. Tetracycline and gentamicin antibiotics were used as positive control and DMSO as negative control, and the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MBC) were determined. One-way analysis of variance and Duncan's test and SAS software were used to compare the mean data.Results : Results: Based on the results of this study, the alcohol extract of all three dose-dependent plants caused a significant increase in the diameter of the zone , preventing the growth of bacteria, especially gram-positive bacteria, compared with the negative control group P(<۰/۰۰۵) Among the extracts studied, ferulago extract had the greatest inhibitory effect on the growth of S.oureus bacteria.(P<۰/۰۵)(۲۵±۰/۵۷)Conclusion : Conclusion: The results of this study showed that all three plants have a growth inhibitory effect on all four bacteria.ferulago extract has more antibacterial activity than other extracts, and Staphylococcus oureus is known to be the most sensitive strain to the extracts used.
کلیدواژه ها:
نویسندگان
Amirreza Akbari
Departement of Microbiology, Ilam Branch,Islamic Azad University,Ilam,Iran
Tayeb Seifi
Veterinary Laboratory Science Expert, Ilam University, Faculty of Veterinary Medicine
Sajjad Mami
PhD in Clinical Pathology, Ilam University, Faculty of Veterinary Medicine
Mohammad reza Nazari
Departement of Microbiology, Ilam Branch,Islamic Azad University,Ilam,Iran
Reza Alizadeh
Departement Veterinary Laboratory Sciences, Ilam University, Faculty of Veterinary Medicine
Bahareh Shah ali
Departement of Microbiology, Ilam Branch,Islamic Azad University,Ilam,Iran