Investigating The Associations of Between Gene Polymorphism NLRP۳ rs۱۰۷۵۴۵۵۸ and IL-۱۸ Expression Level In Patients With Ulcers And Gastritis Caused By Helicobacter pylori

Background and Aim : Helicobacter pylori (H. pylori) is one of the most common bacterial pathogens in humans. The bacterium has been colonized in the stomachs of at least half of the world's population. H. pylori infection is associated with gastrointestinal ulcers, inflammation, and gastric cancer. Several cytokines are secreted from these inflammatory cells, resulting in localized inflammation, which spreads and persists. Interleukin-۱۸ (IL-۱۸) is one of the most important cytokines associated with inflammation of gastric mucosal tissue.The aim of this study was to investigate the expression of IL-۱۸ mRNA in patients with gastritis with H. pylori infection compared to patients with gastric inflammation without H. pylori infection.Methods : In this case-control study, biopsies were collected from ۴۶۴ patients with indigestion referred to the endoscopic ward. The genotype of NLRP۳ rs۱۰۷۵۴۵۵۸ polymorphism was analyzed by RFLP-PCR. Mucosal expression level of IL-۱۸ was measured by Real-Time PCR. The expression of this cytokine in both infected and non-infected groups with H. pylori were statistically analyzed.Results : In current study, IL-۱۸ mRNA expression in biopsy of infected H. pylori patients was significantly higher than non-infected individuals but there was no significant relationship between them.Conclusion : IL-۱۸ may play an important role in the innate immune response to inflammation and the progression of the Th۱ response to H. pylori infection, and play an major role in multiple different clinical outcomes.

کلیدواژه ها:

H. pylori ، IL-۱۸ ، Polymorphism ، NLRP۳ ، Gastritis