Molecular detection of Theileria annulata in dairy cattle of Qazvin province, Iran

Background and Aim : Theileria annulata is one of the most important parasitic diseases in Iran which causes severe economic loss such as the reduction of dairy products. Diagnosis of theileriosis is based on microscpic and clinical examination. Nowadays, molecular detection methods are used for detection such as polymerase chain reactions (PCR) using specific primers. Despite the existence of bovine theileriosis in Iran, no comprehensive study has been done in this matter. So, the aim of this study was detection the cases of T. annulata in dairy cattle from Qazvin province.Methods : A total of ۶۸ dairy cattle with clinical symptoms were taken from blood samples. The blood samples were transported to the laboratory immediately. Then the blood smears were prepared and stained with Giemsa method. Positive samples were incorporated for molecular detection. So, DNA of blood was extracted using phenol-chloroform method and performed PCR using specific primers of Tams۱ and ۱۸SrRNA genes. Vaccine strain of bovine theileriosis and sterile buffer was used as the positive and negative control, respectively. Then, the PCR product of one sample was sent for direct sequencing.Results : The result showed the ring form of Theileria infection in ۲۸ (۴۱.۱۸%) of blood smears. The PCR result indicated that all of the samples were infected with T. annulata. BLAST analyses based on the ۱۸S rRNA gene sequence of the Iranian isolate determined the ۹۹.۱% identity.Conclusion : Based on the obtained results, it is concluded that T.annulata had a high frequency in dairy cattle of Qazvin province.

کلیدواژه ها:

Molecular detection ، Theileria ، annulata ، Qazvin ، dairy cattle